HET-CAM

/Tag:HET-CAM

Results of a Validation Study in Germany on Two In Vitro Alternatives to the Draize Eye Irritation Test, the HET-CAM Test and the 3T3 NRU Cytotoxicity Test

Horst Spielmann, Manfred Liebsch, Sabine Kalweit, Ferdinand
Moldenhauer, Tobias Wirnsberger, Herman-Georg Holzhutter, Berthold Schneider, Sabine Glaser, Ingrid Gerner, Wolfgang J.W. Pape, Reinhard Kreiling, Klaus Krauser, Herbert G. Miltenburger, Winfried Steiling, Nils P. Luepke, Nikolaus Muller, Heinrich Kreuzer, Peter Miirmann, Jochen Spengler, Elke Bertram-Neis, Bernd Siegemund and Friedrich J. Wiebel

You need to register (for free) to download this article. Please log in/register here.
2017-01-09T06:26:06+00:00 Tags: , |

The Mandatory CAM Testing of Cells and Scaffolds for Tissue Engineering: Benefits for the Three Rs of Cooperation with the Vaccine Industry

Erwin Falkner, Claudia Eder, Barbara Kapeller, Walter Fröschl, Christine Schmatz, Karin Macfelda and Udo M. Losert

In cooperation with BAXTER Vaccine AG, which supplies incubated special pathogen-free chicken eggs (including a full veterinary record), a permanent hen’s egg chorio-allantoic membrane test (HET-CAM) unit has been established, where angiogenesis testing, cell culture, and digital and histological analyses are performed. At the Core Unit for Biomedical Research, the location of the animal testing facility of the Medical University Vienna, cell–scaffold constructs must be evaluated in vitro and in ovo prior to eventual in vivo tissue engineering experiments. The animal testing advisory committee requires that new test proposals are first evaluated by using cell culture and HET-CAM models. Approvals for in vivo experiments are postponed and not issued prior to in vitro/in ovo evaluation. Examples are presented of protocols planned for in vivo studies on cell seeded scaffolds, which were refined after in vitro/in ovo evaluations.
You need to register (for free) to download this article. Please log in/register here.