heat-shock protein

/Tag:heat-shock protein

Arsenic Toxicity and HSP70 Expression in Xenopus laevis Embryos

Rosalba Gornati, Claudio Monetti, Davide Vigetti, Stefano Bosisio, Salvador Fortaner, Enrico Sabbioni, Giovanni Bernardini and Mariangela Prati

The evaluation of the effect of trace metals on health can be difficult, because of their presence in the environment in various chemical forms. Exposure to arsenic compounds is an example of this complexity, as it can be present in the environment in inorganic and organic forms. The effects of arsenic in vertebrates are complicated by several variables, such as speciation of the element, the exposure route, and the susceptibility of the particular animal species. The embryotoxicity and teratogenicity of three arsenic species - sodium arsenite (NaAsO2), disodium hydrogen arsenate (Na2HAsO4) and dimethylarsinic acid [(CH3)2AsOOH] - were evaluated by the modified frog embryo teratogenic assay on Xenopus (FETAX). We also show how the classical FETAX endpoints, based on morphological and morphometrical analysis, can conveniently be integrated with the study of molecular markers. Possible changes in the expression of the mRNA for the heat-shock protein HSP70, following exposure to NaAsO2, were examined by using the reverse transcriptase polymerase chain reaction. HSP70 mRNA is strongly induced by arsenic.
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Platinum Toxicity and Gene Expression in Xenopus Embryos: Analysis by FETAX and Differential Display

Claudio Monetti, Giovanni Bernardini, Davide Vigetti, Mariangela Prati, Salvador Fortaner, Enrico Sabbioni and Rosalba Gornati

Since the level of platinum in the environment is destined to increase, because of its use in vehicle catalytic converters, the toxicity of platinum needs further investigation. In this study, the frog embryo teratogenesis assay–Xenopus (FETAX) was used to compare the embryotoxicity and teratogenicity of two common platinum species, (NH4)2PtCl4 and (NH4)2PtCl6. The uptake rates of the two platinum species were studied, and also their effects on the expression of genes encoding metallothionein and heat-shock protein 70, which are known to be induced by several stress factors. In addition, the differential display technique was used to search for genes that were specifically induced by platinum. A gene for the type I collagen α-chain and a novel gene were identified.
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