chicken egg yolk antibodies

/Tag:chicken egg yolk antibodies

The Lipopeptide, Pam3Cys-Ser-(Lys)4: An Alternative Adjuvant to Freund’s Adjuvant for the Immunisation of Chicken to Produce Egg Yolk Antibodies

Michael H. Erhard, Peter Schmidt, Andrea Hofmann, Josefine
Bergmann, Peter Mittermeier, Pia Kaufmann, Karl-Heinz Wiesmüller, Wolfgang G. Bessler and Uli Lösch

This study describes an alternative adjuvant for the immunisation of chicken. The lipopeptide, Pam3Cys-Ser-(Lys)4 (PCSL), has been demonstrated to be a very potent adjuvant, by immunisation with the hapten methyl phosphonic acid paraaminophenyl 1,2,2-trimethylpropyl diester, with coronavirus, with rotavirus and with the Escherichia coli K99 pilus antigen. The antibody titres in chickens that received PCSL were compared with those in animals that received Freund’s complete adjuvant/Freund’s incomplete adjuvant (FCA/FIA). Antigen-dependent differences in antibody titres could be shown. The optimal dosage for PCSL was 250μg per injection. Whereas injection of FCA/FIA resulted in chronic inflammatory alterations, mostly accompanied with granulomatous reactions, no long-term tissue damage could be found with PCSL. When PCSL was used as the adjuvant, the total immunoglobulin Y (IgY) levels in chicken sera were constant during the immunisation period (20.2mg/ml, SD ± 1.3), and were significantly different (p < 0.0001) from those in chickens given FCA as adjuvant (26.6mg/ml, SD ± 4.9). IgY concentrations in the egg yolks showed no significant differences in both groups (PCSL — 13.7mg/ml, SD ± 3.7; FCA — 13.5mg/ml, SD ± 4.2), and were significantly lower (p < 0.0001) than the IgY serum levels.[/fusion_toggle] [/fusion_builder_column][fusion_builder_column row_column_index="1_2" type="1_1" background_position="left top" background_color="" border_size="" border_color="" border_style="solid" spacing="yes" background_image="" background_repeat="no-repeat" padding="" margin_top="0px" margin_bottom="0px" class="" id="" animation_type="" animation_speed="0.3" animation_direction="left" hide_on_mobile="no" center_content="no" min_height="none"][s2If current_user_cannot(access_s2member_level0)] You need to register (for free) to download this article. Please log in/register here.[/s2If]

Evaluation of Various Immunisation Procedures in Laying Hens to Induce High Amounts of Specific Egg Yolk Antibodies

Michael H. Erhard, Katja Mahn, Peter Schmidt, Susanne Oltmer,
Rudolf Preisinger, Pia Zinsmeister and Manfred Stangassinger

The present study, involving 972 laying hens divided into 162 groups (n = 6), was aimed at the development of an immunisation protocol for laying hens to produce specific egg yolk antibodies. Recombinant bovine somatotropin (rbst), Escherichia coli pilus antigen K88 (K88), human serum immunoglobulin G (IgG), and low density lipoprotein (LDL) were used as antigens, each at four different doses (rbst, K88, LDL: 1μg, 10μg, 100μg, 1mg; IgG: 0.5μg, 5μg, 50μg, 0.5mg). Three subcutaneous or intramuscular immunisations were performed at intervals of four weeks. The adjuvant used was either the lipopeptide Pam3Cys-Ser-(Lys)4 (PCSL) or Freund’s incomplete adjuvant (FIA), in two different doses (PCSL: 0.1 and 0.25mg; FIA: 0.1 and 0.25ml). In the four antigen control groups, hens were immunised without any adjuvant. In two negative control groups, only physiological saline was injected. The mean egg weight and egg yield were not influenced by the immunisation procedures. An antigen dose of 10–100g per injection was sufficient to induce high specific antibody titres in the egg yolk. The adjuvant efficacy of PCSL and FIA was proved to be the same (p < 0.05 versus antigen control). With PCSL as adjuvant, some groups showed a tendency to produce even higher specific antibody titres than did FIA groups. A second booster often caused a further significant increase in the amounts of specific antibodies, especially with PCSL. Subcutaneous administration of the antigen together with 250μg PCSL, resulted in a significantly higher immune response than when FIA was used.[/fusion_toggle] [/fusion_builder_column][fusion_builder_column row_column_index="1_2" type="1_1" background_position="left top" background_color="" border_size="" border_color="" border_style="solid" spacing="yes" background_image="" background_repeat="no-repeat" padding="" margin_top="0px" margin_bottom="0px" class="" id="" animation_type="" animation_speed="0.3" animation_direction="left" hide_on_mobile="no" center_content="no" min_height="none"][s2If current_user_cannot(access_s2member_level0)] You need to register (for free) to download this article. Please log in/register here.[/s2If]

The Humoral Immune Response and the Productivity of Laying Hens Kept On the Ground or In Cages

Michael H. Erhard, Haydar Özpinar, Tanay Bilal, Ysmail Abbas, Can Kutay, Hüseyin Eseceli and Manfred Stangassinger

The effects of two different keeping systems on the humoral immune response and productivity were compared for 80 laying hens, divided into four groups. Two groups, each of 20 hens, were kept on the ground and two were kept in cages. All the birds were immunised subcutaneously with human serum immunoglobulin G at a dose of 100μg per injection. The immunisations were performed twice at 4-week intervals. The lipopeptide Pam3Cys-Ser-(Lys)4 was used as an adjuvant at a dose of 0.25mg per injection in one group from each housing system. In the second group from each housing system, the hens were immunised without any adjuvant (antigen control groups). The mean egg yield was significantly higher in both the antigen control group and the adjuvant group, when laying hens were kept in cages. Total egg weight remained constant in both of the housing systems. Keeping hens in cages resulted in higher mean specific antibody titres and mean immunoglobulin Y concentrations in the egg yolk
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