ATLA 40.4, September 2012

//ATLA 40.4, September 2012

Volume 40 issue 4

News & Views

ATLA staff writer

Cancer Research with C. elegans
Dieting Fruit Flies
Ad Libitum Feeding
NATO Countries Still Using Animals for Military Training
Beagle Breeding Facility Temporarily Closed
Model of a Synapse
Bacterial Mutant Screening
Testing the Effects of Pesticides on Human Cells
Microfluidic Platform for Diabetes Research
Comment on REACH
Awards presented at the EUSAAT 2012–Linz 2012 Congress
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CAAT News & Views

ATLA staff writer

Thomas Hartung Now Editorial Board Member of PeerJ Open Access Journal
Paul Locke Appointed to Council of the National Academy of Sciences Institute for Laboratory Animal Research
Martin Stephens Appointed to Board of Chimp Haven
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2017-01-09T06:38:46+00:00

A Pilot Study on the Refinement of Acute Inhalation Toxicity Studies: The Isolated Perfused Rat Lung as a Screening Tool for Surface-active Substances

Monika Fischer, Wolfgang Koch, Horst Windt and Clemens Dasenbrock

New surface-active agents in waterproofing sprays are frequently tested for acute inhalation toxicity in vivo according to OECD Test Guideline 403. In order to refine and reduce the number of acute inhalation tests performed, we propose a screening test that uses isolated lungs. The test consists of the exposure of isolated, ventilated and perfused rat lungs, to aerosolised formulations of waterproofing agents (mass median aerodynamic diameter = 1μm), and on-line monitoring of respiratory parameters and gross pathology analysis. A pilot evaluation of the isolated perfused rat lung model for use in a screening test was carried out by blind testing 12 surface-active substances. The results obtained compared well with data available from in vivo acute inhalation studies. Substances that triggered harmful effects, such as impaired lung compliance and atelectasis of the isolated perfused lung, were also found to cause changes in respiratory parameters, some of which would be severe enough to lead to death in in vivo tests with rats. The changes in respiratory parameters suggest that the mode-of-action is associated with impairment of the surfactant layer. Therefore, pre-testing in the isolated perfused rat lung allows the identification of surface-active substances with the potential for causing acute inhalation toxicity.

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Statistics of Scientific Procedures on Living Animals 2011: Another Increase in Experimentation, but is there a Shiftin Emphasis?

Michelle Hudson-Shore

The 2011 Statistics of Scientific Procedures on Living Animals reveal that the level of animal experimentation in Great Britain continues to rise, with almost 3.8 million procedures being conducted. Unlike those in previous years, this increase is not exclusively due to the breeding and utilisation of genetically altered animals, although they are still involved in the greatest proportion of procedures. That a shift toward fundamental research may have become the primary cause of increases in animal experiments is discussed. The general trends in the species used, and the numbers and types of procedures, are reviewed. In addition, some areas of concern and optimism are outlined.

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The Botulinum Toxin LD50 Potency Assay — Another Chapter, Another Mystery

Andy Pickett

Observers of the potency assay used for botulinum toxin were greeted last year with the news that one company had an alternative, non-animal alternative in place. But all was not as it seemed from the press release, and over a year later, information is still lacking.

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2017-01-09T06:38:47+00:00 Tags: , |

Abstracts of EUSAAT 2012

Various

Progress in 3Rs Research: EU FP6 & FP7 Projects
Chemicals — REACH and Animal Welfare
7th Amendment of EU Cosmetics Directive
Directive 2010/63/EU and Other Legal and Ethical Topics
3Rs Progress in Other Sectors
Inhalation and Nanotoxicology
3R Goes 3D — Implementation of 3D Methods in Toxicity Testing
Free Communications
21st Century Non-animal Tools For Basic and Biomedical Research
Good Cell Culture Practice
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