MaryNew

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About Mary Newman

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So far Mary Newman has created 4 blog entries.

Book Reviews

Various

LABORATORY ANIMAL LAW
DICTIONARY OF TOXICOLOGY
THE WORLD OF INSTINCT
TOXICITY ASSESSMENTALTERNATIVES: Methods, Issues, Opportunities
PROGRESS IN THE REDUCTION, REFINEMENT AND REPLACEMENT OF ANIMAL EXPERIMENTATION

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2017-01-09T06:38:30+00:00 Tags: |

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2017-01-09T06:39:00+00:00

A Phantom Pig Abdomen as an Alternative for Testing Robotic Surgical Systems: Our Experience

Asko Ristolainen,  Gianluca Colucci and Maarja Kruusmaa

The use of animals for testing and validating new medical devices and surgical techniques has raised ethical issues for a long time. Following the introduction of the Three Rs principle, significant efforts have been made to achieve a reduction in the numbers of animals used in testing. Nevertheless, the number of large animals used for testing purposes is still too high. This article describes a potential alternative to the use of large animals in the early phase of the development of surgical equipment — a high-definition phantom pig abdomen. The phantom pig abdomen was developed from computed tomography scans by using affordable materials, and it was used with two different robotic platforms. It permitted the testing of minimally-invasive robotic pancreatic enucleation, with or without intraoperative ultrasound guidance. The phantom pig abdomen has proven to be a realistic tool, with the potential to reduce the cost and time-frame of the experiments.

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2017-01-09T06:39:00+00:00

Preselection of Potential Cancerostatics by Automatic Analysis of Suspended and Adherent Cells Incubated in Microplates

Walter Gutsche and Axel Stelzner

Alternative toxicological screening programmes, without the use of animal experiments, are intended to eliminate dangerous substances and to find new pharmacologically active agents in cell cultures. They can also provide information on the cytostatic activities of the agents. Intercalating cytostatics which bind DNA were selected by measuring the statistical distributions of the cell diameters of K-562 and L-929 cells by using an electronic cell analyser (CASY1). These compounds were identified by cell enlargement or from flat concentration–activity curves created with the cell analyser system. Incubation for 72 hours with DNA-binding agents, such as doxorubicin, daunorubicin and Mitoxantron®, resulted in enlargement of cell diameter and cell volume. The antineoplastic agents actinomycin D and ambazone had no comparable effect. Comparisons of the different parameters obtained with CASY1 measurement were performed with Microsoft EXCEL.
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